Effect of poly(ADP-ribose) polymerase on the response of guinea pig tracheas induced by peroxynitrite in vitro

AIM and METHODS: to elucidater the effect of poly(ADP-ribose) polymerase(PARP) on tracheal hyperreactivity of guinea - pig induced by peroxynitrite, the responses of guinea pig tracheas to histamine af- ter incubation with peroxynitrite in the absence and presence of 3 - aminobenzamide(3 - AB), a highly selective inhibitor for PARP, were observed in vitro. RESULTS: The exposure of tracheal strips to peroxynitrite led to epithelial damage and hyperreacitivity to histamine, both of which were reversed by 3 - AB(lmmol/L or 5mmol/L), whereas incubation of tracheal strips with 3 - AB(5mmol/L) had no effect on the reponses. CONCLUSION: PARP is involved in the epithelial damage and hyperreactivity of guinea - pig tracheas induced by peroxynitrite. The results suggested that inhibition of excessive activation of PARP may represent a novel strategy for the prevention and therapy of airway hyperreactivity in asthma.     

大豆种子抗劣变性的鉴定方法研究

以抗种子劣变性不同的５个栽培大豆和５个野生大豆品种为材料，比较了高温湿（相对湿度１００％，温度４０℃）条件下贮藏３天、７天、１０天和１４天及２０％的甲醇浸泡２小时等５种种子加速第化方法鉴定了种子抗劣变性的效果。结果表明，高温高湿老化７－１０天和２０％的甲醇浸泡２小时，可鉴定出大豆种子抗劣变性。     

CYP450 epoxygenase/EET pathway attenuates adipose inflammation of obese mice through HIF-1α

AIM To investigate the effects of cytochrome P450 （CYP450） epoxygenase/epoxyeicosatrienoic acid （EET） pathway on insulin resistance in obese mice， and to explore the possible mechanisms. METHODS High-fat diet-induced obesity model was established in C57BL/6Cnc mice， and the obese mice were randomly divided into 3 groups， including obesity group （treated with saline； n=10）， EET group （treated with 11，12-EET； n=10） and EET inhibitor 14，15-epoxyeicosa-5（Z）-enoic acid （EEZE） group （n=10）. Normal C57BL/6Cnc mice （n=10） treated with saline served as control. Protein expression of CYP2J2 （one of CYP450 epoxygenases） and hypoxia-inducible factor-1α （HIF-1α） was measured by Western blot. Vessel-like structure was detected by immunofluorescence staining. The serum levels of insulin， tumor necrosis factor-α （TNF-α）， interleukin （IL）-1β， IL-6 and monocyte chemoattractant protein-1 （MCP-1） were measured by ELISA. RESULTS In obese mice， homeostasis model assessment of insulin resistance （HOMA-IR） values were increased， the protein level of CYP2J2 was reduced， and the protein level of HIF-1α was increased in adipose tissues as compared with the controls （P<0.05）. The serum levels of MCP-1， IL-1β， IL-6 and TNF-α were also significantly increased in obese mice （P<0.05）. After treatment with 11， 12-EET， the HOMA-IR values were decreased compared with vehicle-treated obese mice， HIF-1α expression levels were decreased in the adipose tissue， and the serum levels of MCP-1， IL-1β， IL-6 and TNF-α were reduced （P<0.05）. Immunohistochemical results of adipose tissue from vehicle-treated obese mice showed a marked decrease in vessel-like structures （CD31-positive） compared with normal control mice （P<0.05）. EET treatment significantly increased the newly formed vessel-like structures in the visceral adipose tissues of obese mice as compared with vehicle-treated obese mice （P<0.05）. CONCLUSION High-fat diet-induced obesity and insulin resistance are closely related to the CYP450 pathway. Exogenous EETs effectively decrease obesity-induced insulin resistance possibly through pro-angiogenesis and attenuation of hypoxia and inflammation.     

烯效唑对小麦抗冷性影响的研究

研究了不同浓度烯效唑 (S330 7)拌种对早播春型小麦 (川农麦 1号 )抗冷性的影响。结果表明 ,S330 7拌种提高了春小麦四分体期与寒潮期幼穗SOD ,POD酶活力 ,降低了MDA含量 ,同时增加了ABA、可溶性蛋白质和RNA的含量 ,从而提高了植株的抗冷性     

植物阻抗测量的技术方法

植物阻抗是由植物的生理状态和外界刺激因子决定的，通过植物阻抗的测量可以研究植物生理状态和抗逆性。本文分析了植物阻抗的特性，提出了测量方法和应注意的问题。     

稻瘟病菌浅黄色素突变体对稻株诱导抗性的探讨（英文）

经用紫外光诱变菲律宾的一个具有广谱毒性的菌株PO6-6中获得2个浅黄色素突变株A和B以及从浙江省分离的弱致病性菌株C,在25/16℃、RH70%的人工气候箱内,先在4个感病品种上接种48小时后再接种强致病性菌株D和E,浅黄色素突变株和弱菌株对强菌株在水稻感病植株上呈现出交叉保护现象。6批重复试验一致表明,浅黄色素突变株和弱致病性菌株均可明显地诱导水稻植株对稻瘟病的抗病性。本文并就利用稻瘟病菌的交叉保护作用,为开辟防治新途径进行了讨论。     

绵阳系小麦抗条锈性变异的系统调查

 绵阳系小麦是我国重要的抗条锈育种资源,近10年来的田间调查和变异观察圃试验结果表明,绵阳系小麦已经开始丧失成株抗条锈性。田间标样中分离出两类能侵染绵阳系小麦成株的重要致病类群。新的毒性菌系与条中29号相比,毒性谱更宽,致病范围更广,它们是引起绵阳系小麦丧失抗病性的主要原因。新的毒性菌系的出现和发展及绵阳系小麦抗条锈性的丧失,将对我国小麦生产产生重要影响,建议加强绵阳系小麦抗条锈变异的系统监测及控制对策研究。     

抗旱耐热白三叶新品种选育初报

鄂牧一号白三叶是以美国瑞加和路易斯安那两个白三叶品种为原始材料，经单株抗性造反份系比较鉴定，多系混合杂交，品种比较和区域试验选育而成，历时１０年。该品种具有高产，抗旱，耐热和粗蛋白含量高等特性，是南方低山丘陵地区有推广应用前景的优良牧草和草坪植物。     

动物大肠杆菌耐药性的变化趋势

本试验对50年代至90年代初分离保存的50株大肠杆菌进行了药物敏感性测定。结果表明，随着时间的推移，菌株对15种抗菌药物的耐性呈现不同程度的上升趋势，50年代的大肠杆菌分离株对15种抗菌药物均敏感，60年代的分离株对链霉素、四环素产生耐药，70年代对氨苄西林、氯霉素、磺胺甲基异唑、四环素、链霉素、甲氧苄胺嘧啶6种抗生素产生耐药，80年代至90年代初对阿莫西林／奥格门丁、庆大霉素、卡那霉素、萘啶酸、头孢噻吩、氨苄西林、氯霉素、磺胺甲基异唑、四环素、链霉素、甲氧苄胺嘧啶11种抗生素产生耐药，菌株的多重耐药性呈上升趋势。